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Comprehensive quantitative analysis of fatty-acyl ... - ScienceDirect
mouse liver tissue (c57bl/6) was purchased from seralab (sera laboratories international ltd, west sussex, uk) and pulverized in a cryogenic tissue homogenizer (cellcrusher limited, ireland). 10-30 mg of frozen powder was weighed in a polypropylene vial and an appropriate volume of ice cold extraction solvent mixture was added according to .
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(PDF) Pattern Recognition Analysis of Proton Nuclear ... - ResearchGate
Pattern Recognition Analysis of Proton Nuclear Magnetic Resonance Spectra of Brain Tissue Extracts from Rats Anesthetized with Propofol or Isof lurane.pdf Content available from CC BY 4.0:
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Small Laboratory Benchtop Biotech Vacuum Lyophilizer by ...
Buy Small Laboratory Benchtop Biotech Vacuum Lyophilizer from ZhengzhouKedaMachineryandInstrumentEquipmentCo.Ltd Find Company contact details & address in Zhengzhou ...
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Fibers | Free Full-Text | Production of Nanocellulose from ... - MDPI
In this study, the isolation and characterization of nanocellulose from pineapple leaf fibers (PLF) were carried out. Chemical pretreatment included pulping, bleaching, and acid hydrolysis to remove lignin, hemicellulose, and extractive substances were conducted. This was followed by high-shear homogenization and ultrasonication to produce nanocellulose. Morphological changes to the PLF due to ...
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Water Treatment Instruments, Water Treatment Instruments direct from ...
Water Treatment Instruments from AELAB GUANGZHOU CO.,LTD. Search High Quality Water Treatment Instruments Manufacturing and Exporting supplier on Alibaba.
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Cell Culture Flask Price - Buy Cheap Cell Culture Flask At Low Price On ...
Comparing cell culture flask prices. You can easily wholesale quality cell culture flask at wholesale prices on Made-in-China.
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Pattern recognition analysis of proton nuclear magnetic resonance ...
Frozen tissue samples weighing approximately 1.0 g were ground and pulverized into a fine powder in liquid nitrogen using a frozen cell crusher 'Cryo-Press™' (Microtec, Chiba, Japan), and homogenized in 10 ml of a chloroform/methanol (2 1) mixture. After removing the residues by filtration, 1 ml of distilled water was then added to the ...
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Proteomic Alterations Associated with Oral Cancer Patients with Tobacco ...
tissue samples (tumor and adjacent normal) and two pooled samples (for bridging) were labeled. To facilitate cross-cohort comparisons, we utilized two TMT channels (126 and 131) to label a reference sample. This reference sample was generated by pooling equal amount of peptide digest from 12 tumor and adjacent normal tissue pairs and splitting ...
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Sales SCIENTZ-48L high-throughput tissue grinder,Manufacturer
Features: 1. Simple operation Support the centrifuge tube directly into the adapter, support the centrifuge tube direct pre-freezing, the lowest temperature is -50 degrees Celsius freezing. 2. Zero contamination of samples. The grinding process is fully enclosed, and the samples move independently to avoid cross-contamination. 3.
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أسعار أحجام مختلفة للمطاحن الرطبة
أفضل هارد SSD وكيفية اختياره + أسعار هارد SSD. سرعة عالية في نقل البيانات مع أحجام مختلفة; يدعم حماية وتشفير البيانات بمعيار AES 256 bit; سعر هارد SSD من Crucial أرخص من شركات أخرى ; يعتمد على ذواكر ثلاثية الأبعاد لأداء أفضل وسرعة ...
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High Pressure Homogenizer, 12L/h - Xiangyi Instrument
High Pressure Cell Crusher (Homogenizer) Aseptic Homogenizer (slap type) Electric glass homogenate machine. Planetary Ball Mill/ Rotary Tissue Grinder. Tissue Grinder/Ball Mill. Tissue Grinder/Ball Mill. Industry Ultrasonic Homogenizer, 2400W 15KHz. ... Refrigeration & Cryogenic . Freeze Dryer (Lyophilizer) Freezers & Refrigerators ; Circulator ...
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Tissue cryo-pulverization, lysis, and protein extraction 1.1. Fill a dewar with liquid nitrogen. Pre-chill the parts of the tissue pulverizer that will come in contact with the pancreatic tissues, namely the chamber, pulverizer, and recovery spoon in a polystyrene container. ... Cellcrusher: n/a: Used for grinding tissue samples into powder ...
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Cryogenic Processing | CTP Cryogenics
Cryogenic processing is the modification of a material or component using cryogenic temperatures. Lowering stress, improving wear, cutting, strength & more. Get a Free Quote; Have Questions? 818-445-3030. Cryogenic Services. Automotive & Transportation. Engine Builders; How to reduce brake wear;
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Small-Animal PET of Steroid Hormone Receptors Predicts Tumor Response ...
Activity measurements (Bq/cm 3) were divided by the decay-corrected injected dose (Bq) and multiplied by 100 to obtain a tissue uptake index expressed as percentage injected dose per gram of tissue. In Vitro Receptor Assays For Western blot analysis, the excised frozen tumors were crushed with a cryogenic tissue pulverizer (Cellcrusher; Ballina).
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News - LABOAO
The cryogenic coolant circulation pump is a combination of a circulating pump and a cryogenic cooling pump. It is a good auxiliary equipment for vacuum distillation and vacuum concentration. It is especially adapted to be equipped with a rotary evaporator, which not only reduces the use space, but also reduces the vacuum degree and saves water ...
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Thermo Scientific™ National Vial Crushers | Fisher Scientific
Reduce waste disposal costs by reducing the total volume of waste with the Thermo Scientific™ National Vial Crusher. Breaks and separates liquid from all 1mL to 100mL vials and ampules. Capable of processing approximately 5000 2mL vials per hour. Safe use in a fume hood or connect to a vacuum for limited exposure to flammable or toxic vapors.
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（Ningbo Scientz Biotechnology Co.,Ltd ... - Twitter
（Ningbo Scientz Biotechnology Co.,Ltd）. @xinzhishengwuk1. Ningbo Scientz Biotechnology Co.,Ltd, which was founded in 1989 and has carved out a name for itself, is devoted itself in developing and manufacturing instrume. Science & Technology No.65 Mujin Road,Ningbo, China scientzbio Joined July 2022.
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Probing the Hexosamine Biosynthetic Pathway in Human ... - ResearchGate
LC-MS/MS Analysis of Metabolites-Frozen liver tissue (80 -100 mg) was crushed using the CellCrusher TM cryogenic tissue pulverizer under liquid nitrogen, and 1 ml of ice-cold solution of (40%...
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Sitemap - Toption lab
Three layer Glass Reactor 1-3 Liter. Mini Three Layers Glass Reactor 3L for sale. Best lab three layer glass reactor made in China. Three Layer Glass Reactor 1L/5L/10L. Three Layer Glass Reactor 20L/50L/150L. Ultrasonic Homogeneous Reactor SS304L Cylinder Reactor. Graphene Ultrasonic Emulsification Reactor.
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Cryogenic Grinding Seeds | Crusher Mills, Cone Crusher, Jaw Crushers
Cryogenic tissue pulverizers are used to disrupt frozen tissues with a strong extracellular matrix, such as tumours, seeds or venous tissues. Fritsch Pulverisette 23 Mini-Mill is Ideal for Grinding Small . . for wet grinding as well as for dry or cryogenic grinding. . pulping of seeds; Pharmaceuticals and medicine: Kidney and gallstone analysis, .
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Diagnocine Unique Lab Reagents, Consumables, Equipment & Chemicals
Microplate Seal for 96-Well (Cutting Off Type, Horizontal) (100 Sheets) $135.04. View Detail.
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Metabolic Reprogramming by Hexosamine Biosynthetic and Golgi N-Glycan ...
Frozen liver tissue (~100 mg per sample) was pulverized using the CellCrusher TM cryogenic tissue pulverizer under liquid nitrogen. The soluble polar metabolites were extracted by addition of 1 ml ...
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β-Adrenoceptor subtypes and cAMP role in adrenaline- and noradrenaline ...
At the end of the experiment, tissues were rapidly frozen in liquid N 2 to terminate the reaction and crushed using a frozen cell crusher apparatus (Cryo-Press™; Microtec Co., Ltd., Funabashi City, Chiba, Japan). The resulting crushed powders were mixed with 6% trichloroacetic acid (TCA) solution containing 3-isobutyl-1-methylxanthine (IBMX ...
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GI-2C Touch Display Gene Electroporator Intelligent - Xiangyi Instrument
Call us now: +86-731-85421912 Search. Menu
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Comparative analysis of affected and unaffected areas of systemic ...
Frozen skin biopsies were cryo-pulverised to a fine pow- der using a cell crusher device (Cellcrusher, Cork). Skin powdered samples were suspended in a lysis buffer (10 mM Tris-HCl pH7.4; 150mM NaCl; 1mM EDTA; PBS; 0.2% SDS; Proteinase Inhibitor (Roche, USA)) and homogenised by sonication.
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Chronic Diseases Prevention Review - Cancer Cell Research
Take out the preserved spinal cord tissue, add the PBS solution, centrifuge the supernatant under the condition of homogenizing 6 min, 4℃ in the tissue cell crusher for 20 min, operate according to the specification of the kit, add the final solution and mix well. The expression concentration TNF-α 、IL-6 inflammatory factors in
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Probe Sonicator 650W - Ravi Scientific Industries
Probe Sonicator 650W. Make: ACUTEK Model: PRO-650 Power: 650W Adjustment Range: 1-100% Frequency Range: 18-28KHz Frequency tracking accuracy: 1Hz real-time tracking
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Ultrasonic Homogenizer/cell disruptor/ Sonicator
Description: Also known as Sonicator, ultrasonic emulsification machine, ultrasonic dispersion machine, Ideally used in Animal Plant Cells Broken. it can be used for crushing various animals and plants, viruses, cells, bacteria and tissue, also can be used to emulsify, separation, homogenization, extraction, anti-foaming, clear, vegetation nanomaterials, dispersion and speed up chemical ...
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Microtec Co., Ltd.
Frozen Cell Crusher: Cryo-Press, In Cryo-Press, your sample is frozen in liquid nitrogen, and crushed by applying pulsed pressure. Since Cryo-Press prevents thermal denaturation of sample during crushing, It is the best suitable for the DNA, RNA, and protein extraction.Tissues and cells are instantly crushed into fine powder!
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Chemotherapeutic agent 5-fluorouracil increases survival of SOD1 mouse ...
Each muscle was pulverized in liquid nitrogen using a Cellcrusher cryogenic tissue pulverizer (Cellcrusher, Cork, Ireland) and half of the power was kept at -80°C for protein extraction (below). The powered muscle tissue was further homogenized using a PRO200 homogenizer (PRO Scientific Inc) and RNA was extracted with TRIzol reagent (Invitrogen).
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Microtec Co., Ltd. Company Profile
Cell Crushing and Extraction, Universal homogenizer Physcotron can break up to a few microns of cells and the nucleus therein. However, problems such as DNA shredding will occur if the crushing power is too strong. So it is very important to adjust the blade edge according to the object.
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Cell Thawing Protocol for Frozen Primary Cells - STEMCELL
How to thaw frozen primary cells. Human primary cells are cells isolated directly from tissues, including peripheral blood, cord blood, and marrow. These cells are increasingly recognized for their importance in the study of biological processes, disease progression, and drug development, and for applications including in vitro cell-based assays or the creation of xenograft or humanized ...
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BAM15‐mediated mitochondrial uncoupling protects against obesity and ...
Cryo-Electron Microscopy Core, Case Western Reserve University, Cleveland, OH, USA. Search for more papers by this author. ... Frozen tissues were powdered in a tissue pulverizer (CellCrusher, USA) cooled by liquid nitrogen. Powdered tissue samples were homogenized in 90% (v/v) acetonitrile (Sigma-Aldrich, 34851, Australia) and 10% (v/v ...
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Proteomic Alterations Associated with Oral Cancer Patients with Tobacco ...
Apr 8, 2021Introduction. Oral squamous cell carcinoma (OSCC) is the most common form of head and neck cancers with tongue and buccal mucosa being the most reported affected sites (Rivera and Venegas, 2014).According to GLOBOCAN survey 2018, oral cancer is the third most common cancer in India with an incidence rate of 8.9% (Bray et al., 2018).The major risk factors associated with development of oral ...
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Thawing of Frozen Cell Lines - Sigma-Aldrich
In a microbiological safety cabinet, hold a tissue soaked in 70% alcohol around the cap of the frozen ampoule and turn the cap a quarter turn to release any residual liquid nitrogen that may be trapped. Re-tighten the cap. Quickly transfer the ampoule to a 37 °C water bath until only one or two small ice crystals, if any, remain (1-2 minutes).
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Technical Knowledge - News - LABOAO
The cryogenic coolant circulation pump is a combination of a circulating pump and a cryogenic cooling pump. It is a good auxiliary equipment for vacuum distillation and vacuum concentration. It is especially adapted to be equipped with a rotary evaporator, which not only reduces the use space, but also reduces the vacuum degree and saves water ...
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A novel mouse model of hepatocarcinogenesis triggered by AID ... - Nature
Cancerous and non-cancerous liver tissues of approximate volume of 50 mm 3 were macroscopically dissected, frozen in liquid nitrogen and powdered with a frozen-cell crusher (Cryo-press; Microtec ...
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Multiwall carbon nanotubes/polycaprolactone composites for tissue ...
Tissue engineering is an interdisciplinary field that combines the knowledge and technology of cells, engineering materials, and suitable biochemical factors to create artificial organs and tissues, or to regenerate damaged tissues. ... The mixtures were stirred for 6 h on a magnetic stirrer and sonicated for 0.5 h on an ultrasonic cell crusher ...
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